Proteomic Analyses

Analytical Department offers identification of proteins in solutions and gel bands stained with Coomasie or silver. Sample preparation includes reduction, alkylation and enzymatic digestion of proteins.

The resulting mixture of peptides is analyzed by ultrahigh performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and mass spectrum is compared with online databases. Identification is performed by compliance of determined molecular masses of peptides with theoretical masses predicted on the basis of amino acid sequence of proteins.